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Hepa 1-6
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Hepa 1-6 |
Murine Leber Zell-Linie |
400474 |
cryovial |
265,00 |
Hepa 1-6 |
Murine Leber Zell-Linie |
440474 |
vital |
335,00 |
| Designation: | Hepa 1-6 |
| Depositor: | Darlington |
| Organism: | Mus musculus (mouse) |
| Age/Stage: | adult |
| Strain: | C57/L |
| Tissue: | liver |
| Morphology: | epithelial |
| Celltype: | Hepatoma |
| Growth Properties: | monolayer |
| Description: | This cell line is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. The cells can be propagated in a serum free medium consisting of Dulbecco's modified Eagle's medium, 75%; Waymouth's MAB 87/3 medium, 25% plus 3 x10-8 M selenium. Tested and found negative for ectromelia virus (mousepox). |
| Culture Medium: | DMEM:Ham’s F12 supplemented with 2 mM L-glutamine and 5-10% fetal bovine serum. |
| Subculturing: | Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C)until the cells detach (about 2-3 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | twice per week |
| Freeze Medium: | CM-1 (CLS • Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Products: | albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha antitrypsin (alpha-1-antitrypsin); amylase |
References: Darlington GJ et al. Expression of liver phenotypes in cultured mouse hepatoma cells. J. Natl Cancer Inst 64: 809-819, 1980. Darlington GJ. Liver cell lines. Methods Enzymol 151: 19-38, 1987. Jessen KA, Satre MA. Induction of mouse retinol binding protein gene expression by cyclic AMP in Hepa 1-6 cells. Arch Biochem Biophys 357(1):126-30, 1998. Marin JJ, Macias RI, Criado JJ, Bueno A, Monte MJ, Serrano MA. DNA interaction and cytostatic activity of the new liver organotropic complex of cisplatin with glycocholic acid: Bamet-R2. Int J Cancer 78(3):346-52, 1998. Boulares HA, Giardina C, Navarro CL, Khairallah EA, Cohen SD. Modulation of serum growth factor signal transduction in Hepa 1-6 cells by acetaminophen: an inhibition of c-myc expression, NF-kappaB activation, and Raf-1 kinase activity. Toxicol Sci 48(2):264-74, 1999. Seol DW, Chen Q, Zarnegar R. Transcriptional activation of the hepatocyte growth factor receptor (c-met) gene by its ligand (hepatocyte growth factor) is mediated through AP-1. Oncogene 19(9):1132-7, 2000. Liu Y, Wang H, Zhao J, Ma J, Wei L, Wu S, Xie T, Shen F, Trojan J, Habib N, Anthony DD, Wu M, Guo Y. Enhancement of immunogenicity of tumor cells by cotransfection with genes encoding antisense insulin-like growth factor-1 and B7.1 molecules. Cancer Gene Ther 7 (3):456-65, 2000. Zhang BN, Watanabe S, Kohyama M, Saijo K, Kusakabe M, Ohno T. Tumor formation suppressed in gammadeltaT knock-out mice. Cancer Lett 153(1-2):63-6, 2000. Kim JE, Sheen YY. Inhibition of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-stimulated Cyp1a1 promoter activity by hypoxic agents. Biochem Pharmacol 59(12):1549-56, 2000. Su H, Lu R, Ding R, Kan YW. Adeno-associated viral-mediated gene transfer to hepatoma: thymidine kinase/interleukin 2 is more effective in tumor killing in non-ganciclovir (GCV)-treated than in GCV-treated animals. Mol Ther 1(6):509-15, 2000. Matsumura T, Ito A, Takii T, Hayashi H, Onozaki K. Endotoxin and cytokine regulation of toll-like receptor (TLR) 2 and TLR4 gene expression in murine liver and hepatocytes. J Interferon Cytokine Res 20(10):915-21, 2000. Sakai K et al. Physiological differences in insulin-like growth factor binding protein-1 (IGFBP-1) phosphorylation in IGFBP-1 transgenic mice. Diabetes 50(1):32-8, 2001. Park JW, Gruys ME, McCormick K, Lee JK, Subleski J, Wigginton JM, Fenton RG, Wang JM, Wiltrout RH. Primary hepatocytes from mice treated with IL-2/IL-12 produce T cell chemoattractant activity that is dependent on monokine induced by IFN-gamma (Mig) and chemokine responsive to gamma-2 (Crg-2). J Immunol 166(6):3763-70, 2001. Zauner W, Farrow NA, Haines AM. In vitro uptake of polystyrene microspheres: effect of particle size, cell line and cell density. J Control Release 71(1):39-51, 2001. Chang VW, Ho Y. Structural characterization of the mouse Foxf1a gene. Gene 267(2):201-11, 2001. Carrasco J, Criado JJ, Macias RI, Manzano JL, Marin JJ, Medarde M, Rodriguez E. Structural characterization and cytostatic activity of chlorobischolylglycinatogold(III). J Inorg Biochem 84(3-4):287-92, 2001. Wielandt AM, Vollrath V, Farias M, Chianale J. Bucillamine induces glutathione biosynthesis via activation of the transcription factor Nrf2. Biochem Pharmacol 72(4):455-62, 2006. | |
