FS-Balb

Zellname

Beschreibung

Bestell-Nr.

Einheit

Preis, Euro

FS-Balb

Murine Haut Fibrosarkom Zell-Linie

400272

cryovial

315,00

FS-Balb

Murine Haut Fibrosarkom Zell-Linie

440272

vital

395,00



Designation:FS-Balb
Depositor:CLS
Organism:Mus musculus (mouse)
Strain:Balb/c
Tissue:Skin
Morphology:fibroblast
Celltype:fibrosarcoma
Growth Properties:adherent
Description:in vitro established from a primary Sarcoma of Balb/c-mice.
Culture Medium:RPMI 1640 medium supplemented with L-glutamine and 10% fetal bovine serum.
Subculturing:Remove medium, add fresh 0.025% trypsin, 0.02% EDTA solution for 1 to 2 minutes. Remove trypsin and place the flask at 37°C until the cells detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Split Ratio:A ratio of 1:5 to 1:20 is recommended
Fluid Renewal:Every 2 to 3 days
Freeze Medium:CM-1 (CLS - Cell Lines Service)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1


References:

not published

 

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Zertifikat
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