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Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
FS-Balb |
Murine Haut Fibrosarkom Zell-Linie |
400272 |
cryovial |
315,00 |
FS-Balb |
Murine Haut Fibrosarkom Zell-Linie |
440272 |
vital |
395,00 |
| Designation: | FS-Balb |
| Depositor: | CLS |
| Organism: | Mus musculus (mouse) |
| Strain: | Balb/c |
| Tissue: | Skin |
| Morphology: | fibroblast |
| Celltype: | fibrosarcoma |
| Growth Properties: | adherent |
| Description: | in vitro established from a primary Sarcoma of Balb/c-mice. |
| Culture Medium: | RPMI 1640 medium supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.02% EDTA solution for 1 to 2 minutes. Remove trypsin and place the flask at 37°C until the cells detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:20 is recommended |
| Fluid Renewal: | Every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS - Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
References: not published | |