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Lewis-Lung
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Lewis-Lung |
Murine Lungenkarzinom Zell-Linie |
400263 |
cryovial |
265,00 |
Lewis-Lung |
Murine Lungenkarzinom Zell-Linie |
440263 |
vital |
335,00 |
| Designation: | Lewis-Lung |
| Depositor: | CLS |
| Organism: | Mus musculus (mouse) |
| Strain: | C57BL |
| Tissue: | Lung |
| Growth Properties: | Monolayer |
| Description: | Lewis lung carcinoma |
| Culture Medium: | DMEM:Ham' F12 (1:1, vol/vol) supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with EDTA(versene) solution. Add fresh 0.025%trypsin/0.02% EDTA(versene) solution and incubate at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 4 days. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Doubling Time: | 21 hrs |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in C57BL mice |
| Antigen Expression: | H-2b |
| Viruses: | MAP-TEST negative: Sendai, Ektromelia, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theiler's GD VII, Toolan's H-1, MHV, LDV, RCV/SDA, M-Adenovirus, B.piliformis. |
References: Mayo JG et al. Success and failure in the treatment of solid tumors. 3. “Cure” of metastatic Lewis Lung carcinoma with methyl-CCNU (NSC-95442) and surgery-chemotherapy. Cancer Chemother Rep 56: 183-95, 1972. Thompson RB et al. Evaluation of surgery, chemotherapy and immunotherapy on Lewis lung tumour. Rev Eur Etud Clin Biol 17: 900-2, 1972. Mayo JG. Biologic characterization of the subcutaneously implanted Lewis lung tumor. Cancer Chemother Rep2 3: 325-30, 1972. Bertram JS and Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett 11: 63-73, 1980. Axt H. Are there factors of Lewis-Lung-Carcinoma that influence fundamental funtions of mouse macrophages cultivated in vitro? Ph.D. thesis, Berlin, Freie Universität, 2000. URL: http://library.vetmed.fu-berlin.de/diss-abstracts/115952.html. Negishi T et al. NK105, a paclitaxel-incorporating micellar nanoparticle, is a more potent radiosensiting agent compared to free paclitaxel. Br J Cancer 95: 601-6, 2006. | |
