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SVI
Zellname |
Beschreibung |
VE |
Preis, Euro |
|---|---|---|---|
SVI |
Murine Podozyten Zelllinie |
cryovial |
Bitte anfragen |
SVI |
Murine Podozyten Zelllinie |
2xT25 Zellkulturflaschen |
Bitte anfragen |
| Designation: | SVI |
| Depositor: | Dr. N. Endlich |
| Organism: | Mus musculus (mouse) |
| Age/Stage: | adult |
| Strain: | Immorto-Mouse mice; H-2kb-tsA58 |
| Tissue: | kidney |
| Celltype: | podocyte |
| Growth Properties: | adherent; monolayer |
| Description: | The SVI cell line has been cloned from the outgrowth of glomeruli which were isolated from H-2kb-tsA58 transgenic mice. The mice carry a temperature-sensitive variant of the SV40 large T antigen under control of the IFN-g-inducible H-2kb promoter. Cells proliferate at 33°C, and they differentiate at 38°C. At present, the cells have been cultured successfully for more than 40 passages without noting phenotypic changes. |
| Culture Medium: | RPMI 1640 media supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium, wash with PBS; add fresh trypsin/versene solution (0.05% trypsin, 0.02% EDTA) for 3 minutes at 38°C. Add fresh medium, centrifuge and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:3 (38°C) or 1:5 (33°C) is recommended |
| Fluid Renewal: | 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Antigen Expression: | WT-1, Lmx1b, nephrin, NEPHI, FAT, P-cadherin, CD2AP, ZO-I, podocalyxin, podoplanin |
References: Schiwek D. et al. Stable expression of nephrin and localization to cell-cell contacts in novel murine podocyte cell lines. Kidney International 66: 91-101, 2004 Lewko B. et al. Characterization of glucose uptake by cultured rat podocytes. Kidney Blood Press Res 28: 1-7, 2005 Welsch T. et al. Association of CD2AP with dynamic actin on vesicles in podocytes. Am J Physiol Renal Physiol 289: F1134-43, 2005. | |
