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SP2/0-AG14
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SP2/0-AG14 |
Murine Myelom Zell-Linie |
400481 |
cryovial |
265,00 |
SP2/0-AG14 |
Murine Myelom Zell-Linie |
440481 |
vital |
335,00 |
| Designation: | Sp2/0-Ag14 |
| Depositor: | T. Lindl |
| Organism: | Balb/c |
| Tissue: | Hybridoma |
| Morphology: | lymphoblast |
| Celltype: | B cell hybridoma |
| Growth Properties: | suspension |
| Description: | The line was formed by fusing Balb/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma cell line. The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 µg/ml and are HAT sensitive. Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas. |
| Culture Medium: | DMEM supplemented with 4 mM L-glutamine, adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 10% fetal bovine serum. |
| Subculturing: | Maintain cell density between 5 x 104 and 5 x 105 viable cells/ml. |
| Fluid Renewal: | Add fresh medium every 2 to 4 days. |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Antigen Expression: | H-2d |
| Viruses: | Tested and found negative for ectromelia virus (mousepox). |
References: Shulman M et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-70, 1978. Greene GL et al. Monoclonal antibodies to estrophilin: probes for the study of estrogen receptors. Proc Natl Acad Sci USA 77: 157-61, 1980. Ozato K et al. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol 126: 317-21, 1981. Goodrum FD et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J Virol 70: 6323-35, 1996. Chen H et al. Octamer binding factors and their co-activator can activate the murine PU.1 (spi-1) promoter. J Biol Chem 271: 15743-52, 1996. Ota F et al. Serological diversity demonstrable by a set of monoclonal antibodies to eight serotypes of the mutants streptococci. Caries Res 40: 6-14, 2006. Guerin PJ et al. Oxidative stress is not required for the induction of apoptosis upon glutamine starvation of Sp2/0-Ag14 hybridoma cells. Eur J Cell Biol 85: 355-65, 2006. | |
