MH-3924A

Zellname

Beschreibung

Bestell-Nr.

Einheit

Preis Profit, Euro

Preis Non-Profit, Euro

MH-3924A

Ratte Leber Hepatom Zell-Linie

500286

cryovial

468,00

375,00

MH-3924A

Ratte Leber Hepatom Zell-Linie

550286

vital

581,00

465,00



MSDS_MH-3924A

MSDS_MH-3924A.pdf
Designation:MH-3924A
Depositor:CLS
Organism:Rat
Age/Stage:16-month-old rat
Gender:ACI-rat
Tissue:Hepatoma
Morphology:Hepatoma
Growth Properties:monolayer
Description:in vitro established from the Morris hepatoma (CLS Cell lines Service).
RAP-Test negativ
The species was confirmed by Real-Time PCR
Culture Medium:DMEM supplemented with 4.5 g/L glucose, 2mM L-glutamine, and 10% fetal bovine serum (MG-30, CLS order number 820300).
Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37┬░C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Split Ratio:A ratio of 1:4 to 1:6 is recommended
Fluid Renewal:Every 3 to 5 days
Freeze Medium:CM-1 (CLS order number: 800125, 25ml, 800150, 50ml)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:yes, in ACI-rat
Viruses:SMRV negative, as confirmed by Real-Time PCR;
Tested negative in PCR for: Adenovirus FL, Adenovirus K87, Hantavirus, Kilham rat virus, Lmyfocytair choriomeningitis virus, Mycoplasma pulmonis, Pneumonia virus of mice, Rat corona virus / Sialoacryoadenitis virus, Rat parvo virus, Reovirus type 3, Sendai virus, Theiler-s encephalomyelitis virus, Toolan-s H-1 virus.


References:

Chang LO et al. Comparative incorporation of tritiated thymidine and cytidine into the mitochondrial and nuclear DNA and RNA of two transplantable hepatomas (3924A and h-35tc2) and host livers. Cancer Res 28: 2164-7, 1968.
Looney WB et al. Cell proliferation and tumor growth in hepatomas 3924A. Cancer Res 31: 821-5, 1971.
Durban E et al. Purification and characterization of a nuclear DNA-binding phosphoprotein in fetal and tumor tissues. Cancer Res 41: 537-45, 1981.
Zuretti MF et al. Ornithine decarboxylase lability in 2 transplantable highly deviated rat hepatomas. Cancer Lett 36: 35-43, 1987.
Weber G et al. Increased signal transduction activity and down-regulation in human cancer cells. Anticancer Res 16: 3271-82, 1996.
Roberts JP et al. Liver transplantation improves survival of rats bearing hepatoma-3924A. J Surg Res 65: 59-62, 1996.
Schindel DT et al. Hepatic resection enhances growth of residual intrahepatic and subcutaneous hepatoma, which is inhibited by octreotide. J Pediatr Surg 32: 995-7, 1997.
Maataoui A et al. Tranarterial chemoembolization alone and in combination with other therapies: a comparative study in an animal HCC model. Eur Radiol 15: 127-33, 2005.
Vecchini A et al. Dietary PUFA modulate the expression of proliferation and differntiation markers in Morris 3924A hepatoma cells. Biochim Biophys Acta 1737: 138-44, 2005.

 

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