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Zajdela Hepatom
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Zajdela Hepatom |
Ratte Leber Hepatom Zell-Linie |
500306 |
cryovial |
375,00 |
Zajdela Hepatom |
Ratte Leber Hepatom Zell-Linie |
550306 |
vital |
465,00 |
| Designation: | Zajdela-Hepatoma |
| Depositor: | CLS |
| Organism: | Rat |
| Age/Stage: | 11-months-old |
| Strain: | Sprague-Dawley-rat |
| Tissue: | Liver |
| Morphology: | Hepatoma |
| Growth Properties: | monolayer |
| Description: | in vitro established from the Zajdela-Ascites-Hepatoma (Cell lines Service), RAP-Test negative. |
| Culture Medium: | DMEM medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA (versene) solution. Add 0.025% trypsin/0.02% EDTA (versene) solution and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, collect the cells, remove trypsin by centrifugation and dispense into new flasks. Subculture every 3 to 5 days. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | every 3 to 5 days |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in Cörli-rat |
References: Wintzerith M et al. Comparative study of free uridylic nucleotides in the normal liver, the regenerating liver and in the Zajdela hepatoma. CR Seances Soc Biol Fil 156: 2114-8, 1962. Wieser O et al. Heterotransplantation of Zajdela hepatoma of the rat to golden hamsters, mice, and Chinese hamsters. Verh Dtsch Ges Pathol 52: 421-5, 1968. Schnitzler S et al. On the reaction of helix-agglutinins with Zajdela hepatoma cells with regard to the steric arragement of antibodies. Acta Biol Med Ger 21: 877-81, 1968. Atanasov AG et al. Protein conformation and lipid-protein interaction in mitochondria and endoplasmic reticulum membranes of the liver of intact rats and rats with induced Zajdela hepatoma. Dokl Akad Nauk SSSR 200: 1456-8, 1971. Rella W et al. Characterization of membrane antigens from Zajdela-Hepatoma and normal rat liver (author's transl.). Arch Geschwulstforsch. 41: 301-10, 1973. Wojtczak L t al. Effectof glucose and deoxyglucose on the redistribution of calcium in ehrlich ascites tumour and Zajdela hepatoma cells and its consequences for mitochondrial energetics. Further arguments for the role of Ca2+ in the mechanism of the crabtree effect. Eur J Biochem 263: 495-501, 1999. Teriukova NP et al. Detection and identification of a tumor-associated heteroorganic antigen of Zajdela hepatoma in non-histone proteins of chromatin. Tsitologiia 47: 1048-54, 2005. | |
