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DSL-6B/C2
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
DSL-6B/C2 |
Ratten Pankreas Karzinom Zell-Linie |
500167 |
cryovial |
315,00 |
DSL-6B/C2 |
Ratten Pankreas Karzinom Zell-Linie |
550167 |
vital |
395,00 |
| Designation: | DSL-6B/C2 |
| Depositor: | CLS |
| Organism: | Rat |
| Strain: | Lewis |
| Gender: | Male |
| Tissue: | Pancreas |
| Morphology: | Epithelial |
| Celltype: | Pancreatic cell carcinoma; azaserine induced |
| Growth Properties: | Adherent; Monolayer |
| Description: | DSL-6B/C2 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma. The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat(DSL-101-79) that was given azaserine intraperitoneally. The cultured DSL-6B/C2 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture. The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting. |
| Culture Medium: | Waymouth medium supplemented with L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove spent medium and wash once using PBS. Add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at 37°C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Alternatively, Accutase may be used for detaching the cells, 10-15 minutes at 37°C. |
| Split Ratio: | A ratio of 1:3 to 1:4 is recommended |
| Fluid Renewal: | Two times weekly |
| Freeze Medium: | CM-1, cell lines service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in Lewis rats the cells produce solid tumors and partially cystic tumors composed with a mixed phenotype of squamous, mucinous and glandular areas |
| Products: | Mucin. |
References: Pettengill OS, Faris RA, Bell RH Jr., Kuhlmann ET and DS Longnecker. Derivation of Ductlike Cell Lines from a Transplantable Acinar Cell Carcinoma of the Rat Pancreas. Am. J. Pathol. 143(no.1): 292-303, 1993. | |
