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Walker-256
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
Walker-256 |
Ratte Brust Karzinom Zell-Linie |
500375 |
cryovial |
265,00 |
Walker-256 |
Ratte Brust Karzinom Zell-Linie |
550375 |
vital |
335,00 |
| Designation: | Walker 256 |
| Depositor: | CLS |
| Organism: | Rattus norvegicus (rat) |
| Tissue: | Carcinoma |
| Morphology: | Round cells |
| Growth Properties: | Suspension |
| Description: | The Walker cell line has been established from the Walker 256 rat tumor thas has been maintained in vivo for over 60 years. |
| Culture Medium: | Medium 199 or RPMI 1640 medium, supplemented with 2 mM L-glutamine and 5-10% fetal bovine serum. |
| Subculturing: | Dilute in fresh medium to approx. 5x104 cells/ml |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-5 (CLS order no. 800525-25ml or 800550-50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in Cörli rats |
| Viruses: | MAP-test negative for: Sendai, Ektromelie, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theiler`s GD vii, toolan`s H-1, MHV, LDV, RCV/SDA, M- Adenovirus and B.piliformis. |
References: Shetlar MR et al. Serum polysaccharide levels in rats bearing the Walker 256 tumor. Cancer Res 10: 445-7, 1950. Green JW Jr et al. The effect of protein depletion on the host response to transplantable rat tumor Walker 256. Cancer Res 10: 769-74, 1950. Hull RN et al. Establishing long-term cultures of mammalian normal, solid tumor, and ascites tumor cells on glass. Science 117: 223-5, 1953. Schmidt-Gayk H et al. Urinary cyclic AMP and bone histology in Walker carcinosarcoma: evidence of parathyroid hormone-like activity. Eur J Cancer 15: 1211-1218, 1979. Clark CM et al. Actin synthesis and polymerization in the liver of fed and fasted rats bearing a Walker 256 carcinoma. Cancer Res 41: 1973-7, 1981. Arvelo F, Yabrudi A, Delgado ME, González-Cadavid N. Establishment and characterization of cell lines from the Walker carcinoma 256 able to grow in suspension culture and deficient in thymidine kinase. In Vitro. 20(7):549-65, 1984. PMID: 6469274. Fearon KC et al. Reduction of the growth rate of the Walker 256 tumor in rats by rhodamine 6G together with hypoglycemia. Cancer Res 47: 3684-7, 1987. Esbrit P et al. Characterization of parathyroid hormone/parathyroid hormone-related protein receptor and signaling in hypercalcemic Walker 256 tumor cells. J Endocrinology 166: 11-20, 2000. Tandon VK et al. Design, synthesis and evaluation of novel 1,4-naphtoquinone derivatives as antifungal and anticancer agents. Bioorg Med Chem Lett. 14:1079-83, 2004. Zaletok S et al. Role of polyamines in the function of nuclear transcription factor NF-kappaB in breast cancer cells. Exp Oncol. 26:221-5, 2004. Blouin S et al. Relations between radiograph texture analysis and microcomputed tomography in two rat models of bone metastases. Cells Tissues Organs 182: 182-92, 2006. Degasperi GR et al. Verapamil-sensitive Ca(2+) channel regulation of Th1-type proliferation of splenic lymphocytes induced by Walker 256 tumor development in rats. Eur J Pharmacol 549: 179-84, 2006. | |
