COS-7
Cell name |
Description |
Order no. |
Units |
Price, Euro |
|---|---|---|---|---|
COS-7 |
Monkey Kidney Fibroblast cell line |
605470 |
cryovial |
265,00 |
COS-7 |
Monkey Kidney Fibroblast cell line |
665470 |
vital |
335,00 |
| Designation: | COS-7 |
| Depositor: | Gluzmann |
| Organism: | Cercopithecus aethiops (monkey, African green) |
| Tissue: | kidney; SV40 transformed |
| Morphology: | fibroblast |
| Celltype: | fibroblast |
| Growth Properties: | monolayer |
| Description: | The African green monkey kidney fibroblast-like cell line has been established from CV-1 cells which have been transformed by an origin-defective mutant of SV40 coding for wild-type T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40°C, and supports the replication of pure populations of SV40 mutants with deletions in the early region. |
| Culture Medium: | DMEM:Ham's F12 supplemented with 4 mM L-glutamine and 5-10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with fresh 0.02% EDTA solution. Add fresh 0.025% trypsin/0.02% EDTA solution and let the culture sit at 37°C until the cells detach (about 3-5 min). Add fresh medium, resuspend, remove trypsin by centrifugation and dispense into new flasks. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 According to the GenTSV §5 Abs. 2 i.V.m.Anhang Teil B, Teil A II, and the statement of the ZKBS (Central committee for Biological Safety, Germany), the cell line COS-7 is categorized to Biosafety level 1. The COS-7 cell line is corresponds to established monkey cells, which contain defective viral genomes but do not release infectious virus particles to the environment. http://194.95.226.234/GENTEC/ZKBS/ALLGSTELL/90_93/COS.HTM |
| Virus Susceptibility: | SV40 (lytic growth); SV40 tsA209 at 40°C; SV40 mutants with deletions in the early region |
| Applications: | transfection host. Suitable for transfection by vectors requiring expression of SV40 T antigen. |
| Products: | T antigen |
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