CV-1
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
CV-1 |
Affen Nieren Fibroblasten Zell-Linie |
605229 |
cryovial |
265,00 |
CV-1 |
Affen Nieren Fibroblasten Zell-Linie |
665229 |
vital |
335,00 |
| Designation: | CV-1 |
| Depositor: | Jensen |
| Organism: | Cercopithecus aethiops (monkey, African green) |
| Age/Stage: | 141 days |
| Gender: | male |
| Tissue: | Kidney, normal |
| Celltype: | Fibroblast |
| Growth Properties: | monolayer |
| Description: | Derived from the kidney of male African green monkey. |
| Culture Medium: | Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% |
| Subculturing: | Remove media and rinse with EDTA (versene) solution. Add fresh 0.025% trypsin/0.02% EDTA solution for 1 to 2 minutes, remove trypsin and allow the culture to stand at room temperature until the cells detach. Add fresh media, remove trypsin, resuspend in fresh media and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Reverse Transcriptase: | negative |
| Virus Susceptibility: | poliovirus 1; herpes simplex; Eastern equine encephalitis; Western equine encephalitis; California encephalitis; SV40 |
| Applications: | Suitable host for transfection, especially by SV40 vectors. |
References: Proc. Natl. Acad. Sci. USA 53: 53, 1964. Kit S et al. Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid". Virology 27: 453, 1965. Rhoades KL et al. Synergistic up-regulation of the myeloid-specific promoter for the macrophage colony-stimulating factor receptor by AML1 and the t (8; 21) fusion protein may contribute to leukenogenesis. Proc. Natl. Acad. Sci. USA 93: 11895-11900, 1996. Halford WP et al. Mechanisms of herpes simplex virus type 1 reactivation. J. Virol. 70: 5051-5060, 1996. Wu TT et al. Evidence that two latency-associated transcripts of herpes simplex virus type 1 are nonlinear. J. Virol. 70: 5962-5967 .1996. Berson JF et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. Goodrum FD et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. Kolanus W et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. Planelles V et al. Vpr-induced cell cycle arrest is conserved among primate lentiviruses. J. Virol. 70: 2516-2524, 1996. Hill JM et al. In vivo epinephrine reactivation of ocular herpes simplex virus type 1 in the rabbit is correlated to a 370-base-pair region located between the promoter and the 5' end of the 2.0-kilobase latency-associated transcript. J. Virol. 70: 7270-7274, 1996. Dubuisson J and Rice CM. Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. Biardi L and Krisans SK. Compartmentalization of cholesterol biosynthesis. J. Biol. Chem. 271: 1784-1788, 1996. Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. Hocking AM et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. Deleersnyder V et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. | |
