VERO
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
VERO |
Affen Nieren Epitheliale Zell-Linie |
605372 |
cryovial |
265,00 |
VERO |
Affen Nieren Epitheliale Zell-Linie |
665372 |
vital |
335,00 |
| Designation: | Vero |
| Depositor: | Vanderzant |
| Organism: | Cercopithecus aethiops (monkey, African green) |
| Age/Stage: | adult |
| Tissue: | Kidney, normal |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | DMEM:Ham's F12 supplemented with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium, add fresh 0.025% trypsin solution for 1 to 2 minutes, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Reverse Transcriptase: | negative |
| Viruses: | verotoxin detection of virus in ground beef |
| Virus Resistance: | Stratford; Apeu; Caraparu; Madrid; Nepuyo; Ossa |
| Virus Susceptibility: | poliovirus 1, 2, 3; Getah; Ndumu; Pixuna; Ross River; Semliki Forest; Paramaribo; Kokobera; Modoc; Murutucu; Germiston; Guaroa; Pongola; Tacaribe; SV-5; SV40; rubeola; rubellavirus; reovirus 1, 2, 3; simian adenoviruses |
| Applications: | transfection host |
References: Sasaki K et al. Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay. Kitasato Arch Exp Med 37: 27-42, 1964. Simizu B et al. Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). Proc Soc Exp Biol Med 125: 119-23, 1967. Rhim JS et al. Cytopathic and plaque assay of rubella virus in a line of African green monkey kidney cells (Vero). Proc Soc Exp Biol Med 125: 602-6, 1967. Liebhaber H et al. Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero). Proc Soc Exp Biol Med 125: 636-43, 1967. Earley E et al. A plaque neutralization method for arboviruses. Proc Soc Exp Biol Med 125: 741-7, 1967. Rhim JS et al. Cytopathic effects of the parainfluenza virus SV5 in Vero cells. Nature 216: 271-2, 1967. Ozawa Y. Studies on the replication of African horse-sickness virus in two different cell line cultures. Arch Gesamte Virusforsch 21: 155-69, 1967. Rhim JS et al. Growth of Junin virus, the etiological agent of Argentinean hemorrhagic fever, in cell cultures. Arch Gesamte Virusforsch 21: 243-52, 1967. Rhim JS et al. Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc Soc Exp Biol Med 127: 642-6, 1968. Didier ES et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J Eukaryot Microbiol 43: 34-43, 1996. Nichol PF et al. Herpes simplex virus gene expression in neurons: viral DNA synthesis is a critical regulatory event in the branch point between the lytic and latent pathways. J Virol 70: 5476-86, 1996. Govorkova EA et al. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol 70: 5519-24, 1996. White LJ et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J Virol 70: 6589-97, 1996. Martinez R et al. Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates. J Virol 70: 2075-85, 1996. Zeng L et al. Identification of amino acids involved in recognition by dengue virus NS3-specific, HLA-DR15-restricted cytotoxic CD4+ T-cell clones. J Virol 70: 3108-17, 1996. Hill JM et al. In vivo epinephrine reactivation of ocular herpes simplex virus type 1 in the rabbit is correlated to a 370-base-pair region located between the promoter and the 5' end of the 2.0-kilobase latency-associated transcript. J Virol 70: 7270-4, 1996. Carter KL et al. Characterization of the products of the UL43 gene of herpes simplex virus 1: potential implications for regulation of gene expression by antisense transcription. J Virol 70: 7663-8, 1996. Sandri-Goldin RM et al. The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-sm antiserum, and the C terminus appears to be required for this interaction. J Virol 70: 108-18, 1996. Carter KL et al. The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha22 gene. J Virol 70: 172-8, 1996. Russell DW et al. Foamy virus vectors. J Virol 70: 217-22, 1996. Lukonis CJ et al. Characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral DNA replication. J Virol 70: 1751-8, 1996. Lagunoff M et al. Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene. J Virol 70: 1810-7, 1996. Uprichard SL et al. Herpes simplex ICP27 mutant viruses exhibit reduced expression of specific DNA replication genes. J Virol 70: 1969-80, 1996. Leopardi R et al. The herpes simplex virus major regulatory protein ICP4 blocks apoptosis induced by the virus or by hyperthermia. Proc Natl Acad Sci USA 93: 9583-7, 1996. Pereira ME et al. Invasive phenotype of Trypanosoma cruzi restricted to a population expressing trans-sialidase. Infect Immun 64: 3884-92, 1996. Malik AK et al. Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains. J Virol 70: 7859-66, 1996. Chen Y et al. Demonstration of binding of dengue virus envelope protein to target cells. J Virol 70: 8765-72, 1997. Huber M et al. Tyrosine phosphorylation events during coxsackievirus B3 replication. J Virol 71: 595-600, 1997. Pugachev KV et al. Improvement of the specific infectivity of the rubella virus (RUB) infectious clone: determinants of cytopathogenicity induced by RUB map to the nonstructural proteins. J Virol 71: 562-8, 1997. Chomcheon P et al. Cyclopentenones, scaffolds for organic syntheses produced by the endophytic fungus mitosporic dothideomycete sp. LRUB20. J Nat Prod 69: 1351-3, 2006. | |
