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MDBK(NBL-1)
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MDBK(NBL-1) |
Bovine Nieren Zell-Linie |
600396 |
cryovial |
265,00 |
MDBK(NBL-1) |
Bovine Nieren Zell-Linie |
660396 |
vital |
335,00 |
| Designation: | MDBK (NBL-1) |
| Depositor: | Darby |
| Organism: | Bos taurus (bovine) |
| Age/Stage: | adult |
| Gender: | male |
| Tissue: | Kidney (normal) |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The line has been shown to be free of bovine diarrhoea virus. |
| Culture Medium: | Minimum essential medium Eagle with (Earle's BSS) supplemented with 2 mM L-glutamine, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% fetal bovine serum. |
| Subculturing: | Rinse the cell sheet twice with fresh 0.025% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Immunology: | The cells are positive for keratin by immunofluorescence. |
| Reverse Transcriptase: | negative |
| Virus Resistance: | poliovirus 2 |
| Virus Susceptibility: | vesicular stomatitis (Indiana); infectious bovine rhinotracheitis virus; bovine parvovirus; bovine adenovirus I, III; bovine viral diarrhea viruses; parainfluenzavirus 3 |
| Products: | keratin |
References: Madin SH et al. Established kidney cell lines of normal adult bovine and ovine origin. Proc Soc Exp Biol Med 98: 574-6, 1958. Nelson-Rees WA et al. Clonal derivatives of a bovine kidney cell line (MDBK) and continuing preservation of total chromosomal material. Chromosoma 18: 70-8, 1966. Choppin PW et al. The RNAs of infective and incomplete influenza virions grown in MDBK and HeLa cells. Virology 42: 603-10, 1970. Russell DW et al. Foamy virus vectors. J Virol 70: 217-22, 1996. Loffler S et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J Virol 71: 42-9, 1997. Demaegdt H et al. Angiotensin AT(4) receptor ligand interaction with cystinyl aminopeptidase and aminopeptidase N: [125)I]Angiotensin IV only binds to the cystinyl aminopeptidase apo-enzyme. Eur J Pharmacol 546: 19-27, 2006. Barreca C et al. Characterization of a potent refractory state and persistence of herpes simplex virus 1 in cell culture. J Virol 80: 9171-80, 2006. | |
