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MDCK (NBL-2)
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
MDCK (NBL-2) |
Madin-Darby Canine Kidney Zell-Linie |
604280 |
cryovial |
265,00 |
MDCK (NBL-2) |
Madin-Darby Canine Kidney Zell-Linie |
664280 |
vital |
335,00 |
| Designation: | MDCK (NBL-2) |
| Depositor: | Darby |
| Organism: | Canis familiaris (dog) |
| Synonym(s): | canine |
| Age/Stage: | adult |
| Strain: | cocker spaniel |
| Gender: | female |
| Tissue: | Kidney, normal |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products. |
| Culture Medium: | Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% fetal bovine serum. Alternatively, DMEM or DMEM:F12 supplemented with 2 mM L-glutamine and 10% fetal bovine serum may be used. |
| Subculturing: | Before the cells reach confluence, rinse the cell sheet twice with fresh 0.025% trypsin, 0.03% EDTA solution. Remove trypsin, and allow the culture to stand at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Reverse Transcriptase: | negative |
| Virus Resistance: | poliovirus 2; coxsackievirus B3, B4 |
| Virus Susceptibility: | vesicular stomatitis (Indiana); vaccinia; coxsackievirus B5; reovirus 2, 3; adenovirus 4, 5; vesicular exanthema of swine; infectious canine hepatitis |
| Products: | keratin |
References: Gaush CR et al. Characterization of an established line of canine kidney cells (MDCK). Proc. Soc. Exp Biol Med 122: 931-935, 1966. Haass C et al. Polarized sorting of beta-amyloid precursor protein and its proteolytic products in MDCK cells is regulated by two independent signals. J Cell Biol 128: 537-547, 1995. Didier ES et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J Eukaryot Microbiol 43: 34-43, 1996. Mead JR et al. In vitro expression of mRNA coding for a Cryptosporidium parvum oocyst wall protein. J Eukaryot Microbiol 43: 84-85, 1996. von Dippe P et al. The functional expression of sodium-dependent bile acid transport in Madin-Darby canine kidney cells transfected with the cDNA for microsomal epoxide hydrolase. J Biol Chem 271: 18176-18180, 1996. Stuart RO et al. Dependence of epithelial intercellular junction biogenesis on thapsigargin-sensitive intracellular calcium stores. J Biol Chem 271: 13636-13641, 1996. Grindstaff KK et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J Biol Chem 271: 23211-23221, 1996. Loffler S et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J Virol 71: 42-49, 1997. Panneerselvam K and Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J Biol Chem 271: 9417-9421, 1997. | |
