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V79-4
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
V79-4 |
Chinese Hamster Fibroblasten Zell-Linie |
603371 |
cryovial |
265,00 |
V79-4 |
Chinese Hamster Fibroblasten Zell-Linie |
663371 |
vital |
335,00 |
| Designation: | V79-4 |
| Organism: | Cricetulus griseus (hamster, Chinese) |
| Gender: | male |
| Tissue: | lung |
| Morphology: | fibroblastoid |
| Growth Properties: | monolayer |
| Description: | V79-4 is a subline of the parental V79 cells. The V79 cell line was developed by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster, and was originally designated Strain V. Elkind renamed the line V-79 in 1958. The clone V79-4 was isolated by E.H.Y. Chu In 1966, who had obtained the line from W. Sinclair. The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours. They have been intensively used in studies of X-ray induced damage and repair processes. |
| Culture Medium: | DMEM medium supplemented with 2 mM L-glutamine, 4.5 g/L glucose and 5% fetal bovine serum. |
| Subculturing: | Remove medium and rinse with 0.02% EDTA (versene) solution. Add fresh 0.025% trypsin/0.02% EDTA solution and incubate at 37°C for until the cells detach. Add fresh medium, centrifuge, resuspend the cells in fresh medium and dispense into new flasks. |
| Split Ratio: | A ratio of 1:6 to 1:14 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Karyotype: | modal number = 22; range = 20 to 23 Pseudodiploid. The rate of higher ploidies was 4%. Twelve to marker chromosomes were common to most cells. These include 1p-, 4q+, 4p+, t(6,?), 7q+ and seven to eight other small markers. Normal N2 and N3 were paired; N1, N5, N6 and N10 were single. Normal X and Y were absent, but a single Xq- was present in every cell. |
| Reverse Transcriptase: | negative |
References: Ford DK et al. Observation on the chromosomes of Chinese hamster cell in tissue culture. J Natl Cancer Inst 21: 393-425, 1958. Elkind MM et al. X-ray damage and recovery in mammalian cells in vitro. Nature 184: 1293-5, 1959. Chu EH et al. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc Natl Acad Sci USA 61: 1306-12, 1968. Chu EH et al. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62: 359-77, 1969. Sakamoto K et al. Effect of neocarzinostatin on V-79 Chinese hamster cells in vitro. Eur J Cancer 9: 725-8, 1973. Goodrum FD et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J Virol 70: 6323-35, 1996. Chakraborty Sb et al. Prevention and repair of DNA damage by selected phytochemicals as measured by single cell gel electrophoresis. J Environ Pathol Toxicol Oncol 23: 215-26, 2004. Sun YW et al. Sulfonyl-containing nucleoside phosphotriesters and phosphoramidates as novel anticancer prodrugs of 5-fluoro-2’-deoxyuridine 5’-monophosphate (FdUMP). Mol Pharm 3: 161-72, 2006. Novick S et al. Positive cooperativity in the cellular uptake of a boronated porphyrin. Int J Biochem Biol 38: 1374-81, 2006. | |
