Gerne beantworten wir Ihre Fragen
rund um unsere Zell-Linien
und weitere Produkte.
Tel.: +49 (0)6221 700799
Fax.: +49 (0)6221 700717
SF-9
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
SF-9 |
Insekt Ovar Zell-Linie |
604328 |
cryovial |
265,00 |
SF-9 |
Insekt Ovar Zell-Linie |
664328 |
vital |
335,00 |
| Designation: | SF-9 |
| Depositor: | CC-pro |
| Organism: | Spodoptera frugiperda (fall armyworm) |
| Age/Stage: | pupa |
| Gender: | female |
| Tissue: | ovary |
| Morphology: | epithelial |
| Growth Properties: | adherent |
| Description: | For long term culture it is important to use the medium described below. Omission of the TC Yeastolate or lactalbumin hydrolysate will lead to poor performance. |
| Culture Medium: | TC 100 (500 ml) supplemented with 2 mM L-glutamine, 3.3 g of TC Yeastolate, 3.3 g of lactalbumin hydrolysate and 10% heat-inactivated fetal bovine serum. |
| Subculturing: | Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks). If many floating cells are present before subculturing, the old medium and the floating cells may be discarded and the medium replaced before subculture. Incubate the cells at 27°C without CO2. |
| Split Ratio: | A ratio of 1:5 or greater is recommended |
| Fluid Renewal: | 3 times per week |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for Mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Virus Susceptibility: | baculoviruses; Autographa californica (MNPV); St. Louis encephalitis (SLE) |
| Applications: | Transfection host; this line can be used to replicate baculovirus expression vectors. |
References: Vaughn JL et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-7, 1977. Smith GE et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc Natl Acad Sci USA 82: 8404-8, 1985. Zhang PF et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-13, 1994. Hall MR et al. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separately cloned and coexpressed in eukaryotic cells. J Virol 70: 5395-404, 1996. Bakker A et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J Virol 70: 5511-8, 1996. Gibson W et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J Virol 70: 7454-61, 1996. Filtz TM et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J Biol Chem 49: 31121-6, 1996. Lindorfer MA et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J Biol Chem 271: 18582-7, 1996. Bryant SS et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J Biol Chem 271: 5195-9, 1996. Stewart L et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J Biol Chem 271: 7593-601, 1996. Kawabe J et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J Biol Chem 271: 20132-7, 1996. Yasuda H et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J Biol Chem 71: 18588-95, 1996. Chen W et al. Identification of transmembrane domain of a membrane associated protein NS5 of Dendrolimus punctatus cytoplasmic polyhedrosis virus. J Biochem Mol Biol 39: 412-7, 2006. Jin DZ et al. Cryptotanshinone inhibits cylcooxygenase-2 enzyme activity but not its expression. Eur J Pharmacol 549: 166-72, 2006. | |
