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OK
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
OK |
Opossum Nieren Zell-Linie |
606465 |
cryovial |
265,00 |
OK |
Opossum Nieren Zell-Linie |
666465 |
vital |
335,00 |
| Designation: | OK |
| Organism: | Didelphis marsupialis virginiana (opossum) |
| Age/Stage: | adult |
| Gender: | female |
| Tissue: | kidney, cortex |
| Morphology: | epithelial |
| Celltype: | proximal tubule; normal |
| Growth Properties: | monolayer |
| Culture Medium: | Minimum essential medium (Eagle) with Earle's BSS supplemented with 2 mM L-glutamine and 10% fetal bovine serum |
| Subculturing: | Remove medium, add fresh 0.025% trypsin, 0.03% EDTA solution for 2 minutes, rinse and remove. Incubate the flask at 37°C until the cells detach (approximately 5 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A split ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Tumorigenic: | no |
| Receptors Expressed: | alpha2-adrenergic; serotonin; parathyroid hormone; atrial natriuretic factor |
References: Koyama H et al. Establishment and characterization of a cell line from the American opossum (Didelphys virginiana). In Vitro 14: 239-46, 1978. Teitelbaum AP et al. Parathyroid hormone receptors coupled to cyclic adenosine monophosphate formation in an established renal cell line. Endocrinology 114: 980-5, 1984. Yagil C et al. Insulin binding, internalization, and degradation by a cultured kidney cell line. Am J Physiol 254: E601-8, 1988. Cole JA et al. Regulation of sodium-dependent phosphate transport by parathyroid hormone in opossum kidney cells: adenosine 3', 5'-monophosphate-dependent and -independent mechanisms. Endocrinology 122: 2981-9, 1988. Nakai M et al. Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells as a model of renal proximal tubules. Biochem Biophys Res Commun 152: 1416-20, 1988. Cheng L et al. Alpha-2-adrenergic modulation of the parathyroid hormone-inhibition of phosphate uptake in cultured renal (OK) cells. Biochem Biophys Res Commun 155: 74-82, 1988. Hryciw DH et al. Regulation of albumin endocystosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds. Interaction of Na+-H+ exchange regulatory factor- with CIC-5. J Biol Chem 281: 16, 2006. Murtazina R et al. Na+/H+ exchange NHE3 activity and trafficking are lipid Raft-dependent. J Biol Chem 281: 17845-55, 2006. | |
