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PTK-2 (NBL-5)
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
PTK-2 (NBL-5) |
Potoroo Nieren Zell-Linie |
608316 |
cryovial |
265,00 |
PTK-2 (NBL-5) |
Potoroo Nieren Zell-Linie |
668316 |
vital |
335,00 |
| Designation: | PtK-2 (NBL-5) |
| Depositor: | Whalen |
| Organism: | Potorous tridactylis (potoroo) |
| Synonym(s): | Rat kangaroo |
| Gender: | male |
| Tissue: | Kidney, normal |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | Minimum essential medium (Eagle) in reduced bicarbonate (0.85 g/liter) Earle's BSS with with non-essential amino acids, 90%; fetal bovine serum, 10% |
| Subculturing: | Rinse cell sheet 2 times with ATV solution. Remove old medium, let stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Reverse Transcriptase: | negative |
| Virus Resistance: | adenovirus 5; coxsackievirus B5; poliovirus 2 |
| Virus Susceptibility: | coxsackievirus A9; herpes simplex; vaccinia; vesicular stomatitis (Ogden) |
| Products: | keratin |
References: Walen KH. Spatial relationships in the replication of chromosomal DNA. Genetics 51: 915-29, 1965. Lorenz PR et al. Cell cycle analysis and stimulation of rat kangaroo cells (PtK 2)after pulse labelling. Z Naturforsch B 26: 722-4, 1971. Lorenz PR et al. Chromosomes and DNA-replication of rat kangaroo cells (PtK 2). Chromosoma 38: 431-40, 1972. Colombelli J et al. In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery. Traffic 6: 1093-102, 2005. Grunewald TG et al. Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells. Exp Cell Res 312: 974-82, 2006. | |
