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PK-15
Zellname |
Beschreibung |
Bestell-Nr. |
Einheit |
Preis, Euro |
|---|---|---|---|---|
PK-15 |
Schweine Nieren Zell-Linie |
607426 |
cryovial |
265,00 |
PK-15 |
Schweine Nieren Zell-Linie |
667426 |
vital |
335,00 |
| Designation: | PK-15 |
| Depositor: | Armstrong |
| Organism: | Sus scrofa (pig) |
| Synonym(s): | swine |
| Age/Stage: | adult |
| Tissue: | Kidney, normal |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The cells are positive for porcine circovirus (PCV) antigens. The cells are positive for keratin by immunoperoxidase staining. |
| Culture Medium: | Minimum essential medium (Eagle) in Earle's BSS supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10% newborn bovine calf serum |
| Subculturing: | Rinse the cell sheet twice with fresh 0.025% trypsin/0.02% EDTA solution, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS ∙ Cell Lines Service) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Reverse Transcriptase: | positive |
| Virus Resistance: | poliovirus 2 |
| Virus Susceptibility: | hog cholera; African swine fever; vesicular exanthema of swine; foot and mouth disease (FMDV); vesicular stomatitis (Indiana); vaccinia; reovirus 2, 3; adenovirus 4, 5; coxsackievirus B2, B3, B4, B5, B6 |
| Products: | plasminogen activator; keratin |
References: Pirtle EC. Variation in the modal chromosome number of two PK-15 porcine kidney cell lines. Am J Vet Res 27: 747-9, 1966. Pirtle EC et al. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am J Vet Res 29: 153-64, 1968. Armstrong JA et al. C-type virus particles in pig kidney cell lines. J Gen Virol 10: 195-8, 1971. Dulac GC et al. Porcine circovirus antigens in PK-15 cell line and evidence of antibodies to circovirus in Canadian pigs. Can J Vet Res 53: 431-3, 1989. Indik s et al. Isolation and partial characterization of a novel porcine astrovirus. Vet Microbiol 117: 276-83, 2006. Uhlig S et al. Multiple regression analysis as a tool for the identification of relations between semi-quantitative LC-MS data and cytotoxicity of extracts of the fungus Fusarium avenaceum (syn. F. arthrosporioides). Toxicon 48: 567-79, 2006. | |
