U-118 MG

Cell name

Description

Order no.

Units

Price Profit, Euro

Price Non-Profit, Euro

U-118 MG

Human Neuronal Glioblastoma cell line

300362

cryovial

332,00

265,00

U-118 MG

Human Neuronal Glioblastoma cell line

330362

vital

418,00

335,00



MSDS_U-118 MG

MSDS_U-118 MG.pdf
Designation:U-118 MG
Depositor:Ponten
Organism:Homo sapiens (human)
Ethnicity:caucasian
Age/Stage:50 years old
Gender:male
Tissue:Brain
Morphology:mixed
Celltype:glioblastoma (classified as grade IV as of 2007)
Growth Properties:monolayer
Description:This is one of a number of cell lines derived from malignant gliomas (see also U-87 MG, U-138 MG and U-373 MG) by J. Ponten and associates from 1966 to 1969.
Culture Medium:DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine and 10% fetal bovine serum (MG-30, CLS order number 820300).
Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Split Ratio:A ratio of 1:3 to 1:6 is recommended
Fluid Renewal:2 to 3 times weekly
Freeze Medium:CM-1 (CLS order number: 800125, 25ml, 800150, 50ml)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:yes, in nude mice
Karyotype:The line has a near pentaploid chromosome number and a wide range of chromosome number distribution (40% of the cells had numbers ranging from 110 to 115). The following 14 markers were found in most metaphases: t(1p,2p), t(3p,?), t(4p,11q), t(7p,22q), M6, t(9q,?), i(11q)18q t(10q,?), M14, M15, M16, M17 and t(10q,22q); 6 of these were found in some and 10 were seen in one only. Normal chromosomes 7, 8, 12, 19, 20 and 22 had 5 to 6 copies per cell; the X had two copies and the Y was absent.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18
D3S1358: 15
D21S11: 27,32.2
D18S51: 13
Penta E: 7
Penta D: 13
D8S1179: 14,15
FGA: 23
CLS ∙ Cell Lines Service, 2010.
Antigen Expression:Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47
Isoenzymes:Me-2, 1; PGM3, 2; PGM1, 2; ES-D, 1; AK-1, 1-2; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0001


References:

Ponten J et al. Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand 74: 465-86, 1968.
Beckman G et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum Hered 21: 238-41, 1971.
Vaheri A et al. A common cell-type specific surface antigen in cultured human glial cells and fibroblasts: loss in malignant cells. J Exp Med 143: 64-72, 1976.
Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977.
Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977.
Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc Natl Acad Sci USA 75: 3965-9, 1978.
Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981.
Olopade Oi et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-9, 1992.
Cairns P et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res 57: 5356-9, 1997.
Strakova N et al. Peroxisome proliferator-activated receptors (PPAR) agonists affect cell viability, apoptosis and expression of cell cycle related proteins in cell lines of glial brain tumors. Neoplasma 52: 126-36, 2005.
Gross D et al. Platelet-derived growth factor receptor independent proliferation of human glioblastoma cells: selective tyrosine kinase inhibitors lack antiproliferative activity. J Cancer Res Clin Oncol 132: 589-99, 2006.

 

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