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Humane Kolon Adenokarzinom Zell-Linie






Humane Kolon Adenokarzinom Zell-Linie






Organism:Homo sapiens (human)
Age/Stage:58 years; Duke's type B
Tissue:Colon, colorectal
Description:The cells are positive for Colon Antigen 3. They are negative for p53 antigen expression, but positive for mRNA expression. The line was derived from the same tumor as LS 180. LS 174T cells stain positively for cytokeratins.
Culture Medium:Minimum essential medium (Eagle) in Earle's BSS supplemented with 2 mM L-glutamin, 1% non-essential amino acids and 10% fetal bovine serum (MG-10, CLS order number 820100).
Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Split Ratio:A ratio of 1:2 to 1:5 is recommended
Fluid Renewal:2 to 3 times weekly
Freeze Medium:CM-1 (CLS order number: 800125, 25ml, 800150, 50ml)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:yes, in nude mice
Oncogene:myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Karyotype:45,X; one X chromosome missing; no other chromosomal aberrations
DNA Profile (STR):Amelogenin: X,X
CSF1PO: 10,14
D13S317: 10,11
D16S539: 11,13
D5S818: 11,15
D7S820: 11
THO1: 6,7
TPOX: 8,9
vWA: 15,17
D3S1358: 15,17
D21S11: 29,31
D18S51: 11,13
Penta E: 15,16
Penta D: 10
D8S1179: 12,16
FGA: 21,22
CLS ∙ Cell Lines Service, 2010.
Antigen Expression:HLA A2, B13, B50; Blood type O
Isoenzymes:ADA, 1: G6PD, B; PGM1, 1; PGM3, 2; PGD, A; ES-D, 1; PEP-D, 1
Reverse Transcriptase:negative
Products:carcinoembryonic antigen (CEA) 1944 ng/106 cells in 10 days; mucin; interleukin-10 (IL-10); interleukin-6 (IL-6)


Tom BH et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-91, 1976.
Goldenberg DM et al. Antigens associated with normal and malignant gastrointestinal tissues. Cancer Res 36: 3455-63, 1976.
Jakstys MM et al. Process of producing carcinoembryonic antigen. U.S. Pat. 4,228,236.
Chen TR et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet Cytogenet 10: 351-62, 1983.
Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int J Cancer 41: 287-96, 1988.
Gastl GA et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int J Cancer 55: 96-101, 1993.
Shibata D et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat Genet 6: 273-81, 1994.
Kutchera W et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc Natl Acad Sci USA 93: 4816-20, 1996.
Tsao H et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res 58: 109-13, 1998.
Talanov VS et al. Preparation and in vivo evaluation of a novel stabilized linker for 211At labelling of protein. Nucl Med Biol 33: 469-80, 2006.
Nagy P et al. ICAM-1 inhibits the homocluster formation of MHC-I in colon carcinoma cells. Biochem Biophys Res Commun 347: 758-63, 2006.


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