As new product, we offer genomic DNA, free of RNA, prepared from our stock of cell lines:
Bestell-Nummer |
Bezeichnung |
VE |
Preis je VE, Euro |
|---|---|---|---|
300114-D |
Genomic DNA, from A-549 |
20 µg |
145.00 |
300192-D |
Genomic DNA, from HEK293 |
20 µg |
145.00 |
300275-D |
Genomic DNA, from MDA-MB-231 |
20 µg |
145.00 |
300277-D |
Genomic DNA, from MDA-MB-435s |
20 µg |
145.00 |
300483-D |
Genomic DNA, from NCI-H295R |
20 µg |
145.00 |
Please inquire for genomic DNA from cell lines not listed above.
The genomic DNA is isolated by cell lysis followed by the addition of Proteinase K and RNase A and purification on columns. The conditions have been chosen to allow for PCR or other enzymatic reactions in the downstream process.
Applications:
PCR, RT-PCR
Southern Blot
STR analysis
Encymatic reactions
Studies on gene expression.
The fragment length of the purified DNA is up to 50kb.
Quality control:
The quality and quantity of the resulting DNA are determined by measuring the absorption at 260nm /280nm using the Nanodrop 1000.
All data are between 1.8 und 2.0.
As RNAse is added during the isolation, a contamination with RNA can be excluded.
Concentration:
about 100 µg/ml.